By Dr. Mary Bownes
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Additional info for Ecdysone. From Metabolism to Regulation of Gene Expression
C-2 hydroxylation is inhibited by NADPH competitors and by alternate electron ac ceptors, which suggests that the activation of oxygen requires the transfer of electrons to the enzyme. The C-2 hydroxylase showed no affinity for ecdysteroids which are hydroxylated at C-2, such as ecdysone and 20-hydroxyecdysone, indicating that the enzyme has good substrate specificity. Two out of three selective inhibitors of cytochrome P-450-dependent monooxygenases were found to in hibit C-2 hydroxylation. Metyrapone, a competitive inhibitor of steroid monooxygenases in vertebrates, showed an I50 of 60 /iM.
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Ecdysone. From Metabolism to Regulation of Gene Expression by Dr. Mary Bownes