Download e-book for kindle: Ecdysone. From Metabolism to Regulation of Gene Expression by Dr. Mary Bownes

By Dr. Mary Bownes

ISBN-10: 0080320163

ISBN-13: 9780080320168

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C-2 hydroxylation is inhibited by NADPH competitors and by alternate electron ac­ ceptors, which suggests that the activation of oxygen requires the transfer of electrons to the enzyme. The C-2 hydroxylase showed no affinity for ecdysteroids which are hydroxylated at C-2, such as ecdysone and 20-hydroxyecdysone, indicating that the enzyme has good substrate specificity. Two out of three selective inhibitors of cytochrome P-450-dependent monooxygenases were found to in­ hibit C-2 hydroxylation. Metyrapone, a competitive inhibitor of steroid monooxygenases in vertebrates, showed an I50 of 60 /iM.

Pp. 69-82. Elsevier/North Holland. Karlsson P. (1966) Ecdyson, das Hautungshormon der Insekten. Naturwissenschaft 53, 445-453. , Pennetier J. , Modde J. F. and Blais C (1982) Sample processing for high performance liquid chromatography of ecdysteroids. J. Chromat. 236, 137-149. , Van Ballaer E. and Sorgeloos P. (1985) New developments in the high density flow-through culturing of brine shrimp Artemia. A. In press. , Shapiro D. and Shaaya E. (1983) Deter­ mination of ecdysteroid titers by a double-antibody precipitation in the tropical warehouse moth, Ephestia cautella (Lepidoptera, Phycitidae).

1983) Synthesis of high specific activity (23,24)3H42-deoxyecdysone. Nouv. J. Chim. 27, 587-591. Hoffmann J. A. and Hetru C. (1983) Ecdysone. In Endocrinology of Insects (Edited by Downer G. H. ' 65-68. Alan R. Liss, New York. Hsiao T. H. and Hsiao C. (1979) Ecdysteroids in the ovary and the egg of the greater wax moth. J. Insect Phvsiol. 25, 45-52. Johnson P. and Rees H. H. (1977) The mechanism of C-20 hydroxylation of a-ecdysone in the desert locust, Schistocerca gregaria. Biochem. J. 168, 513-520.

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Ecdysone. From Metabolism to Regulation of Gene Expression by Dr. Mary Bownes

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