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82 1 Introduction The developments in molecular and cell biology in the last quarter of the 20th century led to new technologies for the production of complex biomolecules which have the potential to assist in human health care in the areas of diagnostics, prevention and treatment of diseases. One of the most important and at the same time most expensive step in their production is the isolation and cleaning (down-stream processing) of the target biomolecule(s). It represents more than 50% of the total costs of the production process.

They are very effective when a low concentration of the target molecule has to be isolated (captured) from large quantities of raw solution [19]. 3 Monoliths – Continuous Beds Apart from their predominantly diffusive means of mass transport, the problem of particulate separation media is their inability to completely fill the space within the chromatographic column. This also contributes to peak broadening and decreased column efficiency. e. typically a very large cylindrical particle of rigid, highly porous polymer, the void volume can be decreased to a minimum [28, 29].

185. 186. 187. 188. 189. 190. 191. 192. 193. 194. 195. 196. 197. 198. 199. 200. 201. 202. 203. 204. 205. , Teslova 30, 1000 Ljubljana, Slovenia Boehringer Ingelheim Austria GmbH, Dr. Boehringer Gasse 5–11, 1121 Vienna, Austria Monolithic supports represent a novel type of stationary phases for liquid and gas chromatography, for capillary electrochromatography, and as supports for bioconversion and solid phase synthesis. As opposed to individual particles packed into chromatographic columns, monolithic supports are cast as continuous homogeneous phases.

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Capillary Electrochromatography by Svec Frantisek

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